王月丹

• 1:北京大学免疫学系

摘要(Abstract)：

<正>2009年11月27日,农业部批准了两种转苏云金芽孢杆菌(Bt)融合型杀虫蛋白Cry1Ac/Cry1Ab基因的抗虫水稻,"华恢1号"和"Bt汕优63"的安全证书。而

关键词(KeyWords)：

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 王月丹

参考文献(References)：

• [1]农业部农业转基因生物安全管理办公室。农业转基因技术与生物安全问答[D]。http://www.gov.cn/gzdt/2010-03/15/content_1555803.htm
• [2]Schwartz JL,et al.Single-site mu-tations in the conserved alternating-argi-nine region affect ionic channels formed by CryIAa,a Bacillus thuringiensis toxin[J].Appl Environ Microbiol,1997,63:3978-3984.
• [3]Vázquez-Padrón,R.I.,et al.Cry1Ac protoxin from Bacillus thur-ingiensis sp.kurstaki HD73binds to surface proteins in the mouse small in-testine[J].Biochem Biophys Res Comms,2000,271:54-58.
• [4]竺利红,等。苏云金芽孢杆菌Bt9875晶体蛋白诱导HL-60细胞凋亡[J]。微生物学报。48(5):690～694;2008
• [5]殷海成。苏云金芽孢杆菌对黄河鲤白细胞吞噬和溶菌酶活性的影响[J]。河南农业科学。2009,(4):115-117
• [6]European Food Safety Authority.Opinion of the scientific panel on bio-logicalhazards on Bacillus cereusan-dother Bacillus spp in food stuffs[J].The EFSA Journal,2005,175:1-48.
• [7]DAMGAARD P H.Diarrhoeal enterotoxin production by strains of Bacillusthuringiensis isolatedfromcom-mercial Bacillusthuringiensisbased insec-ticides[J].FEMS Immunol Med Microbi-ol,1995,12(2-3):245-250.
• [8]RASKO D A,et al.Genomics of the Bacillus cereus group of organisms[J].FEMS Microbiology Review,2005,29(2):303-329.
• [9]HELGASON E,et al.Bacillus anthracis,Bacillus cereus,and Bacillus thuringiensis-one species on the basis of genetic evidence[J].Appl Environ Mi-crobiol,2000,66(6):2627-2630.
• [10]PERANI M,et al.Prevalence of beta-exotoxin,diarrhoealtoxin and specific delta-endotoxin in natural iso-latesof Bacillus thuringiensis[J].FEMS Microbiol Lett,1998,160(1):55-60.
• [11]YUAN Z M,et al.Detection of enterotoxin genes in mosquito-larvicidal Bacillus species[J].Current Microbiolo-gy,2002,45(3):221-225.
• [12]RIVERA A M G,et al Com-mon occurrence of enterotoxin genes and enterotoxicity in Bacillusthuringiensis[J].FEMS Microbiology Letters,2000,190(1):151-155.
• [13]DAMGAARD P H,et al.En-terotoxinproducing strains of Bacillusthuring-iensis isolated from food[J].Lett Appl Microbiol,1996,23(3):146-150.
• [14]JACKSON S G,et al.Bacillus cereus and Bacillus thuringiensis isolat-ed in a gastroenteritis outbreak investiga-tion[J].Lett Appl Microbiol,1995,21(2):103-105.
• [15]ROSENQUIST H,et al.Occur-rence and significanceof Ba-cilluscereus and Bacillusthur-ingiensis inready-to-eat food[J].FEMS Microbiology Letters,2005,250(1):129-136.
• [16]Malene Schroder,et al.A90-day safety study of genetically modified rice expressing Cry1Ab protein(Bacillus thuringiensis toxin)in Wistar rats[J].Food and Chemical Toxicology;45(2007)339-349.
• [17]Wang,Z.H.,et al.Toxicologi-cal evaluation of transgenic rice flour with a synthetic cry1Ab gene from Bacil-lus thuringiensis[J].Journal of the Sci-ence of Food and Agriculture.2002,82,738-744.
• [18]宋瑞琨,李龙,徐以平。苏云金杆菌内外毒素混合杀虫剂的毒理学研究[J]。同济医科大学学报。1996,25:362-365.
• [19]陈慰峰主编。《医学免疫学》(第四版)[B];人民卫生出版社出版。2004
• [20]Moreno-Fierros,et al.In-tranasal,rectal and intraperitoneal im-munization with protoxin Cry1Ac from Bacillus thuringiensis induces compart-mentalized serum,intestinal,vaginal and pulmonary immune responses in Balb/c mice[J].Microbes Infect,2000,2:885-90.
• [21]Vázquez-Padrón,R.I,et al.Bacillus thuringiensis Cry1Ac protoxin is a potent systemic and mucosal adjuvant[J].Scand J Immunol,1999,49:578-584.
• [22]Vázquez-Padrón,R.I,et al.Intra-gastric and intraperitoneal administration of Cry1Ac protoxin from Bacillus thuringien-sis induces systemic and mucosal antibody responses in mice[J].Life Sciences,1999,64:1897-1912.
• [23]Vázquez-Padrón,R.I.,et al.Characterization of the mucosal and sys-temic immune response induced by Cry1Ac protein from Bacillus thuringiensis HD73in mice[J].Braz J Med Biol Res,2000,33:147-155.
• [24]Bernstein,IL,et al.Immune re-sponses in farm workers after exposure to Bacillus thuringiensis pesticides[J].Environ Health Perspect,1999,107:575-582.
• [25]李芳。转Bt基因玉米种外源蛋白的安全性评价策略及挑战[J]。国外医学卫生学分册。2005,32:96.
• [26]白耀宇,等。转BT基因水稻花粉对中华草蛤成虫产卵和寿命的影响。植物保护学报.2005,32(3):225-230.
• [27]樊龙江,等。转基因BT水稻花粉在桑叶上的自然飘落浓度[J]。生态学报。2003,23(4):827-833.
• [28]舒庆尧,等。转基因水稻“克螟稻”选育[J]。浙江农业大学学报。24(6):579～580,1998.
• [29]Netherwood T,et al.Assessing the survival of transgenic plant DNA in human gastrointestinal tract[J].Nature Biotechnology,2004(22):204-209.
• [30]赵艳,等。转基因植物中标记基因的安全性新策略[J]。遗传。25(1):119～122,2003.